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Hi. I would like to introduce plasmid into Aeromonas hydrophila and Staphylococcus aureus using conjugation. In many articles describing such manipulation were used E.coli S17-1pir or SM10pir as...
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I have performed EMSA on 2% agarose in 0.25 TBE. I can not rid of some unspecific band which is present in whole gel but much stronger in 2 and 3 lanes and its not a shit (shift is on the top of 2...
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