Madison Edwards

4 Questions 8 Answers 0 Followers

Questions related from Madison Edwards

Should a nitrocellulose membrane be used for a negatively charged protein in a dot blot assay?

I would like to do a dot blot assay to test for a negatively charged protein (pI

01 October 2019 2,290 3 View

Do you need salt in column buffer for chitin bead purification?

I would like to purify a protein using the IMACT system where my protein has an intein and chitin-binding domain. I cannot use salt in my purification. The protocol says that you can use 50-1000...

29 August 2018 7,734 3 View

MBP fusion protein not binding to amylose beads?

I have a MBP fusion protein that is not binding to amylose beads. I have already tried adding glucose to the media (although I did not add it to my starter culture) and I am not using detergents...

23 August 2018 5,800 7 View

How to prevent protein aggregation during dialysis to remove imidazole?

I am purifying a 74 kDa protein (about half of the protein is predicted to be disordered) that has an His6-Maltose-binding protein tag (~118 kDa total) total. After eluting from the nickel beads I...

26 April 2018 9,245 6 View