I am trying to do retroviral transduction using platE cells but its not giving good efficiency.Can somebody suggest ways to increase efficiency ?

I use Fugene-3ul/well, pclEco- 0.8ng and polybrene 2ul/ml from 5mg/ml stock. I usually spinfect T cells that I have activated a day before with anti-CD28 and anti-CD3. I use MigR2 plasmid with my...

09 December 2019 3,738 3 View