Madhuri Kanavalli

3 Questions 4 Answers 0 Followers

Questions related from Madhuri Kanavalli

Suggestions to eliminate RNA contamination from human RNA binding protein expressed in E.coli?

I am expressing a human RNA binding protein in E. coli and purifying it using ammonium sulfate precipitation followed by heparin, butyl, and size exclusion chromatography. While the first batch of...

04 April 2023 6,023 3 View

How to design reverse primer with affinity tag, if stop codon is already present in the sequence?

I am trying to design a reverse primer with an affinity tag ( C-term affinity tag). Perhaps, my original protein sequence already has a stop codon at the C term. Could anyone suggest the correct...

16 January 2023 2,604 2 View

Does anyone know the composition of mitochondrial protein extraction buffer / mitochondrial lysis protocol other than sonitation or digitonin?

I am trying to screen the expression of mitochondrial protein from HEK293 cells. Unfortunately, Sonication is not recommended for my protein. Could anyone suggest a protocol for lysis?

06 December 2022 3,390 7 View