Madhura Ketkar

2 Questions 1 Answers 0 Followers

Questions related from Madhura Ketkar

Can a gene re-express after transfecting the cells with lentiviral construct for its shRNA?

I have been trying to stably knockdown a gene in mammalian cells using viral supernatant from HEK293FT cells. Although I have found transfection efficiency to be good enough multiple times, after...

28 August 2021 7,052 1 View

Why do mammalian cells in culture undergo necrosis?

I am observing a large necrotic population in glioblastoma cells that I am culturing in 10% DMEM medium. In spite of changing all parameters of culturing and making sure of the sterilitu, the...

06 November 2020 9,377 3 View