Madhura Bhave

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Questions related from Madhura Bhave

Why do MCF7 spheroids not get formed using the low-adhesion well-plate technique?

I have been trying to culture MCF7 spheroids by seeding 5000 cells in DMEM, in a low-adhesion 96 well-plate (Corning) and then centrifuging at 1000g for 10 mins. However, I am only able to get...

07 March 2017 3,623 4 View

What is the best protocol for subcellular fractionation of Gram-positive bacteria?

I need to obtain the outer cell wall and cytosolic fractions of a Gram-positive bacteria, Clostridium sporogenes. What is the best method of doing so, while ensuring purity of both samples? I want...

12 January 2017 10,092 1 View