Madhu Baghel

3 Questions 3 Answers 0 Followers

Questions related from Madhu Baghel

Which ALP assay protocol is best for mES cells cultured on scaffold ?

I have come across two ways of expressing ALP activity based on the standard curve prepared: (a) using p-nitrophenol standard curve, activity of unknown sample normalised with total DNA (b) using...

04 June 2019 9,532 0 View

What are the methods for identification and absolute quantification of metabolites after 1H-NMR?

I have started doing 1H-NMR for metabolite analysis in biological samples like cell lysate, mice tissue lysate, and mice serum. I am extracting the metabolites using 0.4M Perchloric acid which is...

05 May 2017 9,955 3 View

Can someone suggest me a protocol for detection of Taurine transporter using western blotting in tissue and cell lysates?

I have been trying to detect this protein since long time now. It's a 12 transmembrane containing protein that is of 70 kDa and also glycosylated. It localises in the plasma membrane of cells. My...

13 October 2016 2,176 9 View