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Questions related from Madeline Lewis
I am trying to identify submucosal immune cells in samples of rat gut tissue via IHC, but need a good marker/antibody for these cells. Does anyone have any suggestions as to which marker may be...
07 July 2016 7,361 1 View
I am trying to isolate neutrophils from rat whole blood, aprox. 5 mL collected via cardiac puncture into syringes containing 1mL ACD. I am pipetting whole blood directly onto 5 mL of Histopaque...
06 June 2016 211 5 View
I've been trying to optimize my western blotting protocol and recently started using the mini blot module from life. I ran my blot at 20V for 60 minutes as suggested by the manual and set up a...
05 May 2016 1,687 3 View
I'm trying to use ACK lysis buffer to lyse RBCs from whole blood samples after an initial lysing with H2O (restoring isotonicity with 10% NaCl). My last step is using RIPA buffer + protease...
04 April 2016 5,503 1 View
I am attempting to isolate neutrophils from bovine whole blood and then western blot for l-selectin from neutrophil protein. I am getting bands for bovine l-selectin around 25 kDa, but I've had...
02 February 2016 8,602 0 View
I am trying to isolate neutrophils from bovine and rat whole blood samples. I have purchased the Dynabeads Epoxy and antibody linking kit and have a bovine CD44 and rat NCF2 antibody that can be...
10 October 2015 4,189 2 View
I am trying to identify l-selectin from bovine neutrophils using western blotting, and am having trouble finding an appropriate primary antibody. Any suggestions would be greatly appreciated!
09 September 2015 2,301 1 View
