Madeleine Keefe

6 Questions 3 Answers 0 Followers

Questions related from Madeleine Keefe

Can the addition of glucose for reduction of basal cell expression affect later expression?

I recently got significant functional enzyme produced from a culture of BL21 C41 pLysS cells containing my gene of interest in a pDEST14 vector (the gene codes for a reductase). Functional...

10 October 2019 9,434 4 View

How to activate a metallocarboxypeptidase/metalloexopeptidase?

I am cloning/expressing a gene that makes a metallocarboxypeptidase in BL21-A1 E. coli. However, I know that for this enzyme to be functional it requires a zinc enzyme. I have been looking for...

05 May 2019 322 3 View

Simple lysis buffer for native proteolytic protein expression?

Hello, I have cloned a gene coding for a non-secreted proteolytic protein into E. coli BL21-AI and I am looking for an easy way to lyse these cells in order to extract/purify the protein. I know...

03 March 2019 6,677 4 View

TransDecoder predicted peptide sequences; are the 5-prime partial sequences truly partial?

Hello, I have been using Transdecoder to predict ORFs within my de-novo Transcriptome and to translate nucleotide sequences to their relative peptide sequences. I have been relying on the output...

10 October 2018 7,053 1 View

Alternate ways to clone in an ORF of known sequence?

Hello, I am currently trying to clone and express an ORF of interest with a known sequence that I obtained from a de-novo transcriptome assembly. However, because my original cDNA was sheared in...

10 October 2018 1,965 3 View

Recommended conferences for biotechnology/microbiology/sustainability?

Hello there, I am going to be a third year PhD student with a project that encompasses biotechnology, microbiology, and sustainability. Does anyone have any recommendations for a conference (held...

01 January 1970 7,315 7 View