Madeeha Tariq

10 Questions 3 Answers 0 Followers

Questions related from Madeeha Tariq

What are necessary key point one should consider during subunit computational (MEV) vaccine construction?

Clarify a point for Multiepitope vaccine construction that 1) one should construct 3D structure of MEV with protein adjuvant sequence (A.A) or without it. 2)3D structure refinement, validation,...

07 April 2024 9,844 2 View

For different meats DNA extraction and PCR i need standard ISO, FDA, FAO AOAC latest methods. Can any one provides me these documents?

I need following documents urgently because these document freely not available on ISO web site: ISO 22949-1:2021(MAIN) Molecular biomarker analysis — Methods of analysis for the detection and...

04 March 2023 5,594 0 View

What are the advantages (technical) of Gel documentation system over UV transilluminator?

I am working in a Molecular Biology Laboratory. I already have an UV trans Illuminator, but want to use Gel documentation system. because under transilluminator I experienced ambigious gel results...

25 February 2023 6,991 0 View

For molecular biology testing of raw (mostly contaminated) and cooked meat is it necessary to use BSC II A2?

For raw and cooked meat DNA Extraction and PCR, can I use biosafety cabinet type II in food laboratory?.

19 February 2023 6,147 1 View

Do I need a Biosafety facility (BSC II) for DNA isolation from different raw meat samples and for PCR?

As raw meat microbiological testing and raw meat species, molecular identification is required. is it necessary for raw meat and cooked meat DNA isolation and PCR to avoid cross-contamination and...

16 February 2023 9,683 2 View

Molecular biology for cancer PCR identification and confirmation Biosafety cabinets are used to avoid cross contamination and microbial contamination?

can I use biosafety cabinet for raw/ cooked meat microbiology and meat species molecular PCR based confirmation?

16 February 2023 9,806 0 View

What is the reason of no product in result of PCR but previously in optimization specific gene bands were appear?

i am facing a problem that previously in touch down and in gradient PCR of specific gene, specific band with non specific product come. but after 2-3 weeks when this same gene PCR was performed...

07 October 2021 7,081 3 View

In which concentration and volume (microliters) DMSO 5% or 1-10% I can used to optimize the PCR for specific product to avoid nonspecific product?

In which concentration and volume (microliters) DMSO 5% or 1-10% I can used to optimize the PCR for specific product to avoid nonspecific product? I am facing a problem that I have use 1-10% and...

07 October 2021 9,018 3 View

I am facing the problem o nonspecific product (thick band) and specific product with light band during PCR. what can I do to eliminate this?

during PCR for gradient I used 55C to 68C annealing temperature. Tm of forward and reverse primers was 65.3C and 59.4C respectively. suggest me what can I do? specific product is ~436bp and non...

20 September 2021 4,705 8 View

Is it possible to isolate DNA from animal tissue using chloroform: isoamyl alcohol without phenol? if yes than share reference article or protocol?

I want to extract DNA without phenol and not replacing it with other chemical. And want to use only chloroform and IAA

12 August 2021 7,991 3 View