Madeeha Tariq

5 Questions 2 Answers 0 Followers

Questions related from Madeeha Tariq

What are the advantages (technical) of Gel documentation system over UV transilluminator?

I am working in a Molecular Biology Laboratory. I already have an UV trans Illuminator, but want to use Gel documentation system. because under transilluminator I experienced ambigious gel results...

25 February 2023 6,982 0 View

What is the reason of no product in result of PCR but previously in optimization specific gene bands were appear?

i am facing a problem that previously in touch down and in gradient PCR of specific gene, specific band with non specific product come. but after 2-3 weeks when this same gene PCR was performed...

07 October 2021 7,069 3 View

In which concentration and volume (microliters) DMSO 5% or 1-10% I can used to optimize the PCR for specific product to avoid nonspecific product?

In which concentration and volume (microliters) DMSO 5% or 1-10% I can used to optimize the PCR for specific product to avoid nonspecific product? I am facing a problem that I have use 1-10% and...

07 October 2021 9,005 3 View

I am facing the problem o nonspecific product (thick band) and specific product with light band during PCR. what can I do to eliminate this?

during PCR for gradient I used 55C to 68C annealing temperature. Tm of forward and reverse primers was 65.3C and 59.4C respectively. suggest me what can I do? specific product is ~436bp and non...

20 September 2021 4,694 8 View

Is it possible to isolate DNA from animal tissue using chloroform: isoamyl alcohol without phenol? if yes than share reference article or protocol?

I want to extract DNA without phenol and not replacing it with other chemical. And want to use only chloroform and IAA

12 August 2021 7,988 3 View