Mackenzie Hepker

4 Questions 1 Answers 0 Followers

Questions related from Mackenzie Hepker

Liquid dye in basic histology reagents (like PBS)?

Hi all, I am wondering if there is a colored dye that could be placed in certain liquid histology reagents that come in direct contact with tissue, but would not obstructively bind to it (that...

25 August 2023 5,292 0 View

Does anyone know what this sectioning artifact is?

Frozen sectioning via microtome, sections 40 um thick. I am wondering if it looks like the sections are thinner in those ventral, whiter spots (perhaps from the tissue warming too much in that...

07 December 2022 9,270 4 View

Bubbles forming between frozen section and slide after H2O2 -- any advice?

Hi there, Recently I've been encountering a problem with frozen/fixed-frozen brain blocks embedded in OCT and sectioned with a cryostat. While the sections look fine after mounting them onto...

14 August 2018 7,050 4 View

A classic: tissue falling off charged slides. Help?

Hi all, We're having an issue during IHC with celloidin-treated, paraffin-processed human brainstem tissue sections, one that does not occur with our typical paraffin-processed sections. The...

06 March 2018 9,373 7 View