Maafi Islam

5 Questions 6 Answers 0 Followers

Questions related from Maafi Islam

Why I am seeing a shadow beneath my protein band in a western blot?

Hi, Could anyone please help me understand why a shadow appears beneath my protein band in a western blot? Also, any suggestions for a solution would be greatly appreciated. I have attached an...

29 January 2025 897 4 View

Good Stripping buffer for western blot membrane?

Hello everyone, Does anyone have suggestions on how to achieve complete or almost complete stripping for the western blot membrane? My protein of interest has a molecular weight of 110kda and my...

20 July 2023 691 3 View

Can anyone please recommend a good HNF1-a IP antibody?

Hello everyone! I was looking for a good HNF1-a antibody for western and IP for my mouse intestinal tissue samples. Can anyone please suggest a good antibody? Thank you.

29 June 2022 6,859 0 View

Hello everyone, I was wandering whether there is any other way to look at the phosphorylation status of a protein without doing immunoprecipitation?

Could anyone help me with the following question? I was trying to purify my protein of interest by immunoprecipitation (IP) and look at the phosphorylation status of the protein using specific...

28 April 2022 5,422 2 View

In vitro mouse cell line

Hello everyone, Can anyone suggest me a good mouse cell line that I can use for my in vitro studies? Thank you

01 January 1970 3,558 3 View