Lyle Babcock

3 Questions 13 Answers 0 Followers

Questions related from Lyle Babcock

When designing constructs, do you use the entire sequence of the gene? What sequence do you use?

I'm trying to design a CAR-NK cell that expresses a particular receptor. I have the entire genomic sequence of the gene for that receptor which is 10s of thousands of nucleotides long, I have the...

18 November 2022 2,449 2 View

What is going wrong with my western blot transfers?

Attached is an image of my membrane immediately after the transfer step using a stain-free image. There are no visible protein bands, and the stain-free ladder also didn't transfer. However,...

12 June 2020 3,855 0 View

How do you use 2-NBDG to measure glucose uptake in C2C12 myotubes, with and with out insulin stimulation?

My overall goal is to look at certain membrane components that interfere with insulin-stimulated glucose uptake in muscle fibers (or c2c12 myotubes in this case), so first I have to show an...

17 September 2016 8,257 29 View