Luqi Chen

2 Questions 4 Answers 0 Followers

Questions related from Luqi Chen

Why's my GAPDH clean and my target protein dirty although they are from one same membrane?

I did a western blot yesterday, and I loaded two groups of the same samples in one gel, only separating them when I was incubating the primary antibody. The results show that my GAPDH is clean in...

12 August 2023 3,476 1 View

Why is my site-directed mutation always failed even I do all steps as protocol indicated?

I am an RA doing site-directed mutation in an 8.5kb plasmid; we use the Q5® Site-Directed Mutagenesis Kit from Biolab. I designed my primers in their tool Nebasechanger and followed every step in...

16 May 2023 233 9 View