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Questions related from Luna Vanhorn
Hello, we are developing a protocol for combining FISH and IHC on Drosophila embryonic brain, using FISH to localize mRNA and IHC (2 antibodies) to mark neuropil and neural tracks. We thought...
03 March 2020 3,993 1 View
01 March 2020 3,388 3 View
I did a bacteria transformation and ended up with low transformation efficiency for all my plates. Here's the protocol I used following addition of competent cells to the plasmids and mixing, and...
11 November 2019 6,226 12 View
Below is the gel image I got following a blue-white colony screening and colony PCR. I got the right bands for my inserts in lanes 2&3 but got only fuzzy, non-specific bands of roughly the same...
25 October 2019 3,559 8 View
