Luke Prescott

3 Questions 1 Answers 0 Followers

Questions related from Luke Prescott

If western blot densitometry values are logarithmic, why are target protein values normalized to loading controls only with division?

I have been imaging western blots on a LI-COR Odyssey Fc. After looking into what the densitometry really means, I'm confused as to how only dividing the target protein's density by the same...

08 August 2016 9,296 1 View

What is the best way to combine technical and biological replicates?

I have been running western blots with multiple biological replicates (separate protein isolations from cells treated the same way) each with multiple technical replicates (different lanes...

08 August 2016 7,775 1 View

How can old or reused western blot antibodies cause more variability in the normalized fold change data between blots?

I have been running many western blots and have not been able to always use fresh primary antibodies each use because of a limited supply. The proteins were isolated from cells treated with...

08 August 2016 3,715 3 View