Luke Nelson

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Questions related from Luke Nelson

Do you think the C Terminal P2A peptide (GSGATNFSLLKQAGDVEENPG) would affect Rab27a function?

I have made a plasmid that translates Rab27a-P2A in mammalian cells. Rab27a's C Terminus (CXC) has a critical motif for post translation geranylation. This modification allows for vesicle...

15 May 2025 8,459 0 View

Does Unicorn 5.31 workstation require a separate disk for strategy installation?

I am setting up an AKTA purifier with Unicorn 5.31 (Cytiva, full version). I’ve reached the point in the installation where it requests a strategy for upload. The workstation doesn’t come with 2...

14 August 2021 9,039 0 View

Why Aren't My 1L Bacterial Cultures Growing Properly?

I performed a fresh transformation (with sequenced plasmid) into commercial BL21 competent cells. I scrape a colony into a starter culture w/ antibiotic overnight. I check the O.D. of the starter...

16 October 2018 3,313 9 View