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Questions related from Lukasz Lagojda
Good morning everyone, I am currently trying to analyse my blots using the Gels tool in ImageJ software. In the attachment provided, there are three outputs of the same membrane after selecting...
09 September 2018 7,952 0 View
Hello, I am having problems with beta actin in some of my samples. These are cerebellar samples under denaturing conditions, 10ug protein per well. Transfer looks fine after Ponceau S but beta...
05 May 2018 315 2 View
So these are my saline-treated cerebellum samples. Lanes 1-9 are loaded with equal amount of protein. This membrane was stained for b-actin only, and as you see some bands are missing (my protein...
03 March 2018 5,185 3 View
I perfused some mice for IHC last week and found today that the pH of PFA was not adjusted to 7.4 (the pH is 11.3) Is there anything I can do to save these brains and use them further for IHC or...
11 November 2017 6,642 6 View
