Lukas Hyka

3 Questions 2 Answers 0 Followers

Questions related from Lukas Hyka

How to achieve high expression of my protein in mammalian stable cell line?

Hi, I am working with HEK293 cells and I am trying to express a Cas9 fusion (about 220 kDa) under the control of the EF-1α core promoter. The issue I am facing is that the expression of Cas9...

27 April 2023 5,082 3 View

Does anyone have experience with using qEV columns by Izon for EV isolation?

Hi, we are trying to set up the qEV columns for EV isolation and we are experiencing difficulties with reproducibility. Prior to loading the EVs we concentrate using Amicon filters. Once we have...

22 November 2021 8,126 3 View

How do you store GW4869 (inhibitor of exosome release)?

Hi, I am using the GW4869 in my experiments and I am wondering about the best storing conditions. I would like to store the GW4869 resuspended in DMSO in aliquots of 5mM. Do you have any idea if...

22 March 2021 6,610 3 View