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Questions related from Luis Fernando Garcia-Alles
Dear people, I would like to integrate the coding DNA for an additional chaperone somewhere within the sequence of the PG-KJE8 (from Takara company, sold by Clonetech). I wonder whether any of you...
04 April 2018 9,352 1 View
Hi, I am looking for an expression plasmid to be used in combination with pET15b or pET26b. I need to express two potential protein partners in E coli. I prefer to do the tests with two vectors,...
04 April 2018 9,198 0 View
Dear people, I just would like to hear your opinion on the posible meaning of the next observation. I am presently trying to purify a protein that is suspected to assemble with other protein...
03 March 2018 6,234 6 View
I seek for advice on the next point: I need to coexpress two proteins from two independent plasmids, normaly in BL21(DE3). The first one would be a pET15b or pET26b-like one (pBR322 ori)....
01 January 1970 9,251 7 View