Luis Fernando Garcia-Alles

3 Questions 3 Answers 0 Followers

Questions related from Luis Fernando Garcia-Alles

Expression vector compatible with pET15b or pET26b?

Hi, I am looking for an expression plasmid to be used in combination with pET15b or pET26b. I need to express two potential protein partners in E coli. I prefer to do the tests with two vectors,...

12 April 2018 4,849 1 View

DNA sequence of pG-KJE8?

Dear people, I would like to integrate the coding DNA for an additional chaperone somewhere within the sequence of the PG-KJE8 (from Takara company, sold by Clonetech). I wonder whether any of you...

03 April 2018 1,599 1 View

Plasmid combination for controled independent coexpression of two proteins in E. coli

I seek for advice on the next point: I need to coexpress two proteins from two independent plasmids, normaly in BL21(DE3). The first one would be a pET15b or pET26b-like one (pBR322 ori)....

01 January 1970 9,992 6 View