4 Questions 3 Answers 0 Followers
Questions related from Luis Fernando Garcia-Alles
Hello, I would like to run MD on a protein with a porphyrin-Fe cofactor. The YASARA-automatized semi-empirical treatment of the cofactor is not working properly when launching MD with the AMBER14...
21 March 2023 7,359 0 View
Hi, I am looking for an expression plasmid to be used in combination with pET15b or pET26b. I need to express two potential protein partners in E coli. I prefer to do the tests with two vectors,...
12 April 2018 4,889 1 View
Dear people, I would like to integrate the coding DNA for an additional chaperone somewhere within the sequence of the PG-KJE8 (from Takara company, sold by Clonetech). I wonder whether any of you...
03 April 2018 1,652 1 View
I seek for advice on the next point: I need to coexpress two proteins from two independent plasmids, normaly in BL21(DE3). The first one would be a pET15b or pET26b-like one (pBR322 ori)....
01 January 1970 10,045 6 View
