Luigi Nicassio

4 Questions 4 Answers 0 Followers

Questions related from Luigi Nicassio

Has someone some experience in working with the kit "ATP Bioluminescence Assay Kit CLS II" (Sigma-Aldrich)?

In particular I need to know if it is possible to use frozen homogenate tissues as starting material. All the protocol I found talk about cell coltures. Thank you very much.

07 July 2017 4,876 1 View

Which kind of buffer can I use to resuspend trans,trans-Farnesyl pyrophosphate ammonium salt?

I have to use trans,trans-Farnesyl pyrophosphate ammonium salt as a substrate in an enzymatic assay. I bought this product from SIGMA...

19 April 2017 5,692 1 View

Which kind of buffer can I use to resuspend a lyophilized Pectinase enzyme?

I bought a Pectinase enzyme from Sigma in a powder form (http://www.sigmaaldrich.com/catalog/product/sigma/17389?lang=it®ion=IT), but I can't find any datasheet that shows how to resuspend...

30 March 2017 518 2 View

Western blot normalization for olive fruit extract: do you know a typical housekeeping protein used for WB on plants and specifically on Olives?

Hi all. I need to evaluate Polyphenol oxidase (PPO) protein content in olive fruit extracts by Western blot. So I'm searching also a housekeeping protein in Olea europea for normalization. Some...

09 January 2017 5,416 3 View