Ludia Pack

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Questions related from Ludia Pack

Kidney preparation for cryosectioning?

I have been using the following protocol for preparing mouse kidneys for cryosectioning: 1. Perfusion with 4% PFA 2. Fixation O/N in 4% PFA 3. 30% sucrose O/N 4. 30% sucrose O/N 5. Embedding in...

29 June 2022 2,695 2 View

What does it mean when PJ694A or alpha loses its characteristic red phenotype, and does it have implications for the viability of the ADE2 reporter?

I unfroze some new cells from these strains in order to have fresh colonies to start some new Y2H screens with. However, these cells have lost the redness that the original strains had. I plated...

29 October 2018 3,366 3 View

Can you add 3AT directly to pre made SD plates?

I am looking for a quick fix because I am pressed for time. I know that you can add ampicillin directly onto LB plates, and was wondering if the same would apply to 3AT plates. I could not find...

26 July 2018 6,156 2 View