Anyone have experience with collagenase assay not working when isolating LPMCs?

We have diluted the collagenase II at 0.1ug/ml in PBS, first using a pre digest buffer of 5mM EDTA in HBSS. The cells are in the pre digest for two 20 minute incubations, washed and then added to...

02 February 2014 226 2 View