Lucinda Gaelejwe

2 Questions 2 Answers 0 Followers

Questions related from Lucinda Gaelejwe

Does sodium azide have a toxic potential when using in the lab?

Am doing a DBS eluteion and in it i will have to add PBS and Tween and Sodium azide. Does sodium azide have any toxicity in it. and what measures of precaution should i take.

06 June 2016 3,954 3 View

How do I troubleshoot for the KOD Hotstart DNA polymerase kit?My PCR products are stuck in the wells of the gel. The Tm of the primer is 47 degress.

I have optimized the annealing temperature from 50-47 degress. I have also optimized the volume of DNA added from 1-5 ul but the PCR products are still stuck in the well.

05 May 2016 9,860 3 View