Has anyone stained pSTAT3 in T cells for FACS?

I am stimulating with IL-27, IL-6, or IL-21 and trying to see pSTAT3 in naive CD4+ T cells. I have tried BD's protocol using the Perm buffer III, I have used Stumhofer et al., 2007, AOH Hall et...

04 November 2020 2,784 3 View