Lucia Schoderboeck

2 Questions 3 Answers 0 Followers

Questions related from Lucia Schoderboeck

Where does a single nucleotide ambiguity in sequencing of a digested plasmid, that was not in the original plasmid, come from?

We have been having issues with this certain plasmid for a while: we were trying to remove one gene (GFP) and replacing it with another. The digest of the original plasmid with BamHI and NotI...

12 August 2019 8,422 8 View

What causes high background signal in IHC specifically at AAV injection site?

We are injecting mouse brains with an AAV delivering a transgene. When we then try to use anti-mouse secondary antibodies, we get high background specifically in the region that has been injected....

14 May 2018 6,948 4 View