Where does a single nucleotide ambiguity in sequencing of a digested plasmid, that was not in the original plasmid, come from?

We have been having issues with this certain plasmid for a while: we were trying to remove one gene (GFP) and replacing it with another. The digest of the original plasmid with BamHI and NotI...

12 August 2019 8,338 8 View