Lucas Oliveira Guerra

3 Questions 4 Answers 0 Followers

Questions related from Lucas Oliveira Guerra

Why my cells (or DNA) are turning into a gel during my DNA extraction?

When I'm proceeding my DNA extraction protocol, my DNA becomes an insoluble gel in solution and it's impossible to measure the concentration. The cells that I'm using are Human Keratinocytes...

08 August 2019 517 0 View

How can I hydrolyses my DNA extraction?

I'm trying to hydrolyses my DNA that I extracted from HaCat cells and analyse by HPLC. When I proceed the protocol with a control sample (Sigma Calf DNA) I can see the 4 bases in the UV spectrum,...

08 August 2019 5,293 3 View

How can I treat my cells w/ aldehyde (acrolein) and let they in incubator withou harm the other cells that are in the incubator too?

When I treated my cells with acrolein (less then 1mM) (in a 12 well cell culture cluster) the other cells that were in a tissue culture flask 25 (growing normally without any treatment) were...

04 April 2019 7,611 4 View