Luca Magnani

3 Questions 4 Answers 0 Followers

Questions related from Luca Magnani

Is it possible to create stable cell lines with a plasmid not carrying mammalian selection by co-tranfection of a second plasmid with resistance?

Hi all. I have one reporter plasmid and need to create a stable cell line. The plasmid lacks mammalian selection and I was planning to build one in. A colleague however suggested that...

02 February 2014 9,434 20 View

SPRI select reagent kit vs ampure xp

Has anybody tested SPRI select reagents vs. Ampure XP for size selection? Also, I have seen a couple of thread that suggested moving size selection AFTER PCR amplication. My assumption is that...

07 July 2013 3,391 1 View

Which is the best strategy for a large cloning project?

We are embarking in a medium-large scale project. We want to clone cDNAs (full size when < 10kb) of 50-100 genes. Next, we want to use these amplicons for targeted re-sequencing. 2 questions: 1-...

06 June 2013 1,555 4 View