Luca Bruni

3 Questions 7 Answers 0 Followers

Questions related from Luca Bruni

Are there cell fixation protocols suitable for the studies of cell morphology and cytoskeleton with AFM?

I usually use PFA 4%. However I must fix cells (Hs 578Bst) with a not hazard-toxic compound. I thought to use air flow comes of biological cape, but the morphology must be affected. Some...

20 April 2017 489 4 View

Has anyone performed an immunostaining reaction of actin stress fibres on Hs 578Bst/HTB-125 human cell line?

I'm interested in comparing AFM topography images with images coming from actin stress fibers immunostained on Hs 578Bst/HTB-125 cell line. So I’m wondering if someone could give us just on image...

15 December 2014 8,971 4 View

Which is the best oxidant to get ferric (Fe3+) hemin: acetylphenylhydrazine or phenylhydrazine?

I'd like to get hemin Fe 3+, to fold DNAzyme with all hemin molecules in an oxidated state, to avoid some catalytic effects on some components making folding buffer. I want to use...

03 November 2014 3,973 2 View