Lorcan Carlsen

3 Questions 1 Answers 0 Followers

Questions related from Lorcan Carlsen

Can the temperature of the SOC outgrowth medium in bacterial transformation cause cell lysis/aggregation?

I have done many bacterial transformations over the past few years without any major problems. I use NEB 5-alpha high efficiency cells for transformations with plasmids, according to the NEB...

29 April 2022 261 3 View

Is it possible to lose your gRNA and Cas-9 sequences within the binary vector, in Agrobacterium tumefaciens LBA4404?

I am trying to develop a binary construct containing a gRNA sequence and a Cas9 coding sequence, to transform Agrobacterium, to subsequently transform tomato plants and knock out a target gene....

10 April 2021 2,667 1 View

Can you substitute MS media for LB media in agroinfiltration media in tomato?

I've designed several CRISPR-Cas9 binary vector constructs and I'd like to test them in tomato plants via agroinfiltration first, to confirm that they successfully cleave the genomic DNA before...

30 September 2019 898 4 View