Liya Liang

3 Questions 1 Answers 0 Followers

Questions related from Liya Liang

Did anyone use the SC-ADE medium for selection and if I mutate or delete the ade2 gene, should I get red colonies in the SC-Ade medium plate?

I bought the medium SC-ADE and mutated the ade2 gene. I want to know more information about this gene selection and how long can I see the red colonies and is there anything I should notice? Thank...

20 October 2015 1,529 1 View

How might I amplify a long fragment from a plasmid?

I want to amplify a fragment from a plasmid and the length is about 5700bp. And I designed my primer with 25bp homologous to my plasmid and 60bp tail at both sides. I tried many conditions but I...

09 June 2015 883 6 View

Why doesn't my gibson assembly work?

I am using the NEB HiFi DNA Assembly Master Mix to assemble 4 fragments (about 1000bp for each) to pUC19(2700bp). The total length is about 7500bp. But I tried several times, I didn't get any...

01 June 2015 2,139 5 View