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Questions related from Liuba Cherkashchenko
Good day. Help me, please understand how to split adherent cells (Vero)....For example, I have T25 flask with cells and i need to split at the ratio 1:15 ("based on the surface area")?
08 October 2019 7,532 2 View
Good day! I work with Cowpox virus (BAC). I did transfection (with Wild type). Used FuGene for that. After 24 h i could not detect any plaque formation. What is a problem may be? Concentration of...
21 November 2016 9,083 1 View
So, the problem is that I deleted 2 genes essential for plaque formation (I am talking about Poxvirus - Cowpox virus).I found information that Poxvirus can replicate only in the RK13 but without 2...
18 October 2016 3,499 3 View
Good day! I have a question. This is my first time that I am working with a primary antibodies. What can I use as a diluent for this? PBS?
09 October 2016 9,412 14 View
