Liu Bin

4 Questions 11 Answers 0 Followers

Questions related from Liu Bin

How to do siRNA knockdown and lenti virus overexpression transfection togther?

Hi, guys, I want to study two genes' function in endothelial cells at the same time. I would like to use siRNA to knock down one gene and lentivirus to overexpress another gene. Do you have any...

19 July 2021 9,083 3 View

Why my glycolysis seahorse data looks so strange?

Hi, guys, recently I am performing the glycolysis seahorse assay in endothelial cells. I followed the protocol as suggested, but the ECAR didn't increase after adding Oligomycin. Do you think the...

19 July 2021 3,241 4 View

Does DNA methylation have effect on RNA methylation, how about the underlying mechanism?

Due to that the DNA methylation and the RNA methylation could both regulate the gene expression, so here is my confusion: does the DNA methylation have the effect on RNA methylation? For example,...

23 April 2019 336 2 View

How can I predict and detect protein phosphorylation sites with other kinases?

I want to know whether a new protein is phosphated by other kinases or not. And there is no such phosphorylation antibody to detect, so how could I know whether the protein could be phosphorylated...

10 June 2015 8,791 12 View