Liselotte Mackens

5 Questions 2 Answers 0 Followers

Questions related from Liselotte Mackens

CD3-coated 96-well plate - CD3 did not work. Storage/expiration?

I recently performed an experiment where I coated a CD3 antibody on a 96 well (flat-bottom, TC treated) plate at a concentration of 4 µg/ml (50 µl per well) for 2h at 37°C, 5% CO2. Afterwards, I...

17 January 2023 1,888 2 View

Low viability of isolated naive CD4+ T-cells in culture (24h) and how to improve this?

Hello, I am currently optimizing a naive CD4+T-cell activation protocol based on plate-bound CD3 and CD28 (both 4 µg/ml) in a TC 96-well plate. I use RPMI (2 mM L-glutamine) + 10% FBS + 1%...

07 October 2021 4,494 3 View

T-cell leukemia/lymphoma cell with with xCT expression?

Hello, I am looking for a T-cell like cell line (like Jurkat) that expresses xCT (system xc-, SLC7A11). Could anybody please recommend any cell lines (+refs if possible) that I could use? Kinds...

08 March 2021 2,916 1 View

How long do mouse CD4+ T-cells remain activated after removal of CD3/28?

Hello, I would like to analyse naive CD4+ T-cell activation and intracellular processes with a machine that does not allow CD3-coated plates. I thought of activating my cells with plate-bound...

09 December 2020 3,630 1 View

Sample size needed for robust data about T-cell subpopulations?

I would like to set up a pilot study to investigate the role of a certain receptor in the development and maintenance of several T-cell subtypes (both CD4+ and CD8+ as well as various forms of...

22 September 2020 3,390 1 View