Why coating with fibronectin (1mg/ml or 200ug/ml) does not significantly improve HUVEC attachment to PES/PVP membranes?

I coated PES/PVP membranes with 1mg/ml and 200ug/ml human fibronectin (30min, 37C) after which HUVECs were seeded on top. I try to improve attachment to the membrane and have found numerous...

01 December 2015 5,284 5 View