Lisandro Alfonso Pacheco

4 Questions 11 Answers 0 Followers

Questions related from Lisandro Alfonso Pacheco

What are the advantages of using small-inhibitor molecules vs siRNA inhibition to trace endocytic routes?

I know that the siRNA inhibition is chronic and the small-molecule inhibition is temporary and reversible. Do you have an explanation related to this question?

01 January 2013 6,515 5 View

RNA-seq: 454 or Illumina?

I want to profile miRNA's in plasma of patients with lupus nephritis through Next Generation Sequencing. I have been reading about the advantages both Illumina and 454 in RNA-seq.

12 December 2012 8,692 9 View

How to calculate if an inhibitor "X" has an effect on the virus entry in cell culture?

I am trying to determine if an inhibitor "X" is blocking the early events in a virus "Y". The cell culture, incubation with the inhibitor and the virus infection are standarized in the lab. I run...

02 February 2012 6,810 16 View

¿How to estimate the cell confluency in culture flask/plates?

Hi everybody. I usually estimate the cell confluency in culture flasks/plates in a subjective way, looking the cells and then calculating what is the area they are populating. ¿Do exist a...

02 February 2012 5,935 17 View