Lisa Walz

3 Questions 2 Answers 0 Followers

Questions related from Lisa Walz

How can I detect phospho HSL (Ser600) with westernblot? I cannot find any antibody for Ser600?

Hello all, I would like to investigate whether my molecule activates lipolysis via PKC. The PKC activates the MAPK pathway which phosphorylates the hormone sensitive lipase (HSL) at Ser600....

27 August 2021 7,112 4 View

Which passage of 3T3-L1 cells is the best for differentiation and at what point are the cells too old?

I am currently working with 3T3-L1 cells. From which passage do the cells differentiate best and from when should I rather thaw new cells because the passage is too high? Is there a...

18 March 2021 7,862 2 View

How to perform a lipolysis experiment with 3T3 L1 cells?

Hello, I am currently working with 3T3 L1 cells and allowing them to differentiate into 24 well plates. After that, I would like to stimulate lipolysis with forskolin and sphingosine-1-phosphate...

24 February 2021 5,665 5 View