Lindsey Dale Goodman

2 Questions 2 Answers 0 Followers

Questions related from Lindsey Dale Goodman

How many nucleotides does RNAPII bind at one time on the template DNA?

In other words, what # of nucelotides does RNAPII span during transcription of a gene? (ie. 5 nucleotides? 25 nucleotides?)

31 October 2017 3,890 1 View

Why do most cell lysis buffers for western have either NP40 OR Triton-X? Is there a reason that you can't mix the two into one universal buffer?

I want to resolve both membrane and cytoplasmic proteins from the same sample. In my hands, the membrane-bound protein is best resolved with NP40-containing buffer (150mM NaCl) while the...

12 April 2016 6,764 2 View