Why do most cell lysis buffers for western have either NP40 OR Triton-X? Is there a reason that you can't mix the two into one universal buffer?

I want to resolve both membrane and cytoplasmic proteins from the same sample.  In my hands, the membrane-bound protein is best resolved with NP40-containing buffer (150mM NaCl) while the...

12 April 2016 6,682 2 View