How do I stop RIPA-insoluble, Urea-soluble protein fraction from precipitating in Western blot wells?

I’m trying to analyze insoluble proteins from mouse brain. Basically, I extract with RIPA, then take the pellet that’s left over and extract with a urea buffer (7M urea, 2M thiourea, 4% CHAPS, 30...

11 November 2015 1,967 8 View