Lily Wang

3 Questions 5 Answers 0 Followers

Questions related from Lily Wang

How can I improve the quality of my plasmid?

Dear all, I am now working on in vitro transcription but struggled with low yield. One of my friends told me that the quality of plasmid, especially the proportion of supercoiled plasmids, has...

23 January 2025 4,231 3 View

Why is the yield of my in-vitro transcription so low? and every time lower than before?

Hi everyone, I am working on in-vitro transcription to generate mRNA for LNP fabrication. The kit I am using is the HiScribe® T7 mRNA Kit with CleanCap® Reagent AG from NEB. The first time I was...

15 January 2025 2,995 6 View

How to avoid cell loss when wash with PBS for 2 times in 96 well plate？

Hi, I am working on establishing PC12 and bEnd.3 OGD/R model in 96plate. Because the nutrient substrate in full culture medium is somehow influence the oxygen/glucose deprivation process, I...

01 January 1970 6,701 0 View