Liana Oliveira

5 Questions 7 Answers 0 Followers

Questions related from Liana Oliveira

How do I concentrate RNA or cDNA?

We extracted RNA from blood cells to analyze gene expression. We now have an aliquot with 500ng of the extracted RNA to make the reverse transcription (RT). The problem is that this 500ng are in a...

23 September 2015 7,749 14 View

How can I increase reads (OD values) on ELISA tests?

I am using an ELISA kit to detect BAFF on human serum. The kit has a standard curve that goes from 0.31ng/ml to 20ng/ml. In the literature, I found no article that used the exact same kit, but...

08 January 2015 3,998 3 View

How can I improve standard curves for ELISA kits?

I am using an ELISA kit to measure APRIL in human serum. This kit provides a liofilyzed standard that we have to reconstitute and make dilutions in the plate. The curve is supposed to go from 0.78...

08 January 2015 9,397 5 View

Does anybody uses BAFF and APRIL eBiscience ELISA kits?

I have only a minimum experience on ELISA tests and I am trying to use eBioscience kits for BAFF (BMS2007INST) and APRIL (BMS2008) and I'm not having good results. I was wondering if someone who...

04 January 2015 5,686 4 View

How can I calculate the odds ratio, CI, and P values when I have a null value?

I'm working on a case-control study and I'm using VassarStats (http://vassarstats.net/odds2x2.html) to estimate the OR, 95% CI, and to perform the two-tailed Fisher's exact test. The problem is...

05 November 2014 3,760 8 View