Li-Lian Yuan

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Questions related from Li-Lian Yuan

What is the optimal method for isolating extracellular vesicles from plasma while balancing both yield and purity?

In extracellular vesicle research, various isolation methods exist, including ultracentrifugation, PEG precipitation, size exclusion chromatography, and commercial kits. Could anyone share...

14 March 2025 1,871 0 View

Which company offers the best estrogen receptor alpha (ERα) antagonist for in vivo animal studies in terms of quality and affordability?

We are looking to purchase an estrogen receptor alpha (ERα) antagonist for in vivo animal studies, and ICI 182,780 appears to be a good choice. Tocris offers 50 mg of ICI for $1,119, while...

13 March 2025 3,265 1 View

What is the optimal protocol for preparing extracellular vesicle (EV) samples for LC-MS/MS-based proteomics?

We have isolated extracellular vesicles (EVs) from plasma samples and are preparing them for LC-MS/MS-based proteomics at a core facility. However, I am uncertain whether EV lysis is necessary...

12 March 2025 8,863 3 View