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We usually do PEI preciptation to move nucleic acid and ammonium sulfate precipitation before purificating a certain protein,then resolve the precipitate with binding buffer (20mM Tris pH...
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I am working on AAV purification, and we are facing the problem that the host cell DNA of the final product is relately high, more than 100ng/dose.we guess that the host cell DNAs is enveloped...
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