Letícia Castro

4 Questions 8 Answers 0 Followers

Questions related from Letícia Castro

Does anyone ever try to use MspI restriction enzyme to digest bovine DNA?

I'm trying to use the Premium RRBS kit (Diagenode) to analyze bovine sperm samples and apparently the restriction enzyme digestion (MspI) is not working for sperm samples, it looks completely...

16 May 2018 4,177 6 View

Does anyone try to perform MNase digestion in bovine sperm?

I'm trying to digest bovine sperm DNA with MNase for ChIP-Seq but I can't get the nucleosome band (around 150 bp) in the agarose gel. I followed the Hisano et al. protocol (Genome-wide chromatin...

07 December 2017 1,800 3 View

How can I remove the extender from bovine thawed semen?

I need to remove the extender from the straws without lost too many cells. I don't need the spermatozoa viable because after the separation I will remove the tail and plasma membrane and perform...

24 June 2017 6,374 8 View

How can I dissolve proteins after TCA/acetone precipitation?

I'm using the following protocol (https://www.ncbi.nlm.nih.gov/pubmed/25193639) to precipitate proteins from sperm nucleus: - 500 ul of the sample + 125 ul of 100% TCA (final concentration...

31 May 2017 8,104 4 View