Lelamekala Vengidasan

5 Questions 18 Answers 0 Followers

Questions related from Lelamekala Vengidasan

I produce CRISPR gRNA by using PCR method. I obtained 10ug of RNA from invitro transcription.Why I can see any band in gel electrophoresis after IVT?

I produce CRISPR gRNA by using PCR method. I obtained 10ug of RNA from in-vitro transcription. But when I proceed to gel electroporesis analysis I can see any visible band. There was almost blank....

20 June 2016 6,769 5 View

Which is the best pair of gRNAs that I should for my CRIPSR single site correction?

I'm going to do point mutation correction by using CRISPR Ribonucleoprotein method and ss-ODN. Guide-RNA design softwares from the internet gave me few possible guideRNAs. I'm planning to use two...

12 April 2016 8,536 3 View

Can I use the normal Quikchange Kit for large plasmid 10Kb?

Quikchange Kit II (PfuUltra HP DNA polymerase) I would like to introduce point mutation in the plasmid.

20 January 2016 7,768 3 View

Why does the Colony PCR show a positive result, but the digestion shows negative?

Vector : pet26(+) 5360bp Insert : 1500bp RE : NdeI and XhoI After digestion, I elute out single band from both vector and insert and continue with ligation using Quick Ligation form NEB....

02 April 2015 4,914 34 View

Is there any kit to silence my gene up to 90% that you guys would recommend, as most kits available can only silence up to 60-70%?

I would like to silence the G6PD gene in CD34+ progenitor cells. I would like to use shRNA to silence my gene up to 90% by using lentiviral viral transduction. Are there any kits that you guys...

10 June 2014 8,125 2 View