Lelamekala Vengidasan

4 Questions 17 Answers 0 Followers

Questions related from Lelamekala Vengidasan

I produce CRISPR gRNA by using PCR method. I obtained 10ug of RNA from invitro transcription.Why I can see any band in gel electrophoresis after IVT?

I produce CRISPR gRNA by using PCR method. I obtained 10ug of RNA from in-vitro transcription. But when I proceed to gel electroporesis analysis I can see any visible band. There was almost blank....

20 June 2016 6,747 5 View

Which is the best pair of gRNAs that I should for my CRIPSR single site correction?

I'm going to do point mutation correction by using CRISPR Ribonucleoprotein method and ss-ODN. Guide-RNA design softwares from the internet gave me few possible guideRNAs. I'm planning to use two...

12 April 2016 8,513 3 View

Can I use the normal Quikchange Kit for large plasmid 10Kb?

Quikchange Kit II (PfuUltra HP DNA polymerase) I would like to introduce point mutation in the plasmid.

20 January 2016 7,739 3 View

Why does the Colony PCR show a positive result, but the digestion shows negative?

Vector : pet26(+) 5360bp Insert : 1500bp RE : NdeI and XhoI After digestion, I elute out single band from both vector and insert and continue with ligation using Quick Ligation form NEB....

02 April 2015 4,884 34 View