Leela Sai Lokesh

3 Questions 4 Answers 0 Followers

Questions related from Leela Sai Lokesh

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

24 February 2021 7,988 2 View

What is the maximum size of spheroid we can obtain from Caco-2 cell line?

Hi all i am trying to develop spheroids using Caco-2 cell line. I used Ultra low attachment plates from corning where i added 50k cells/ well and found 0.8-1.0 mm cell aggregate on 3rd day. My...

07 February 2019 7,753 0 View

What is the role of EDC and NHS in conjugating folic acid with chitosan ?

i am using EDC and NHS along with folic acid to prepare folic acid - chitosan conjugation. i would like to know the role of EDC and NHS in this reaction

26 July 2018 8,634 4 View