Lee Yee Seng

7 Questions 6 Answers 0 Followers

Questions related from Lee Yee Seng

In linkage genetics (between 2 genes), is it possible to obtain negative LOD scores? Isn't it do not make sense with negative linkage?

The recombination frequency of 2 unlinked genes is 50% (independent assortment, which is maximal achievable recombination frequency. For linked genes, their θ should be 0 < θ < 0.5 (not...

23 April 2020 7,603 0 View

What is the suitable ammonium sulphate % for fractional precipitation of ATP-citrate lyase whereby that % doesnt precipitate complex I resp chain?

I am having a problem when assaying ATP citrate lyase from the crude extract of Y. lipolytica (continuous assay) that is the exogenous activity is too high. Based on literature, I suspect it is...

19 February 2019 5,930 4 View

Can I know why is Yarrowia lipolytica considered oleaginous even though its wild type strains rarely accumulate lipid more than 20% dry cell weight?

I am so confused as I found out that some sources mentioned Y. lipolytica can accumulate lipid up to 36% of DCW. But there are also some sources mentioned that the wild-type strain of the yeast...

12 February 2019 6,711 4 View

Hi all, can I know what are the possible reasons that there is no spontaneous formation of bilayer in folch method for lipid extraction?

I am trying to extract lipid from Y. lipolytica. I had done sonication of the cells suspended chloroform-methanol （2:1). But after addition of 1% NaCl, some of the samples form bilayer...

10 February 2019 3,586 4 View

Hi all, if I am to assay ATP-citrate lyase by coupling with malate dehydrogenase, can I add rotenone or amytal to enhance the accuracy of my assay?

I am having a problem by assaying ATP-citrate lyase (ACL) continously. I had the validation with and without cell free extract (CFE) in my reaction mixture. The result is that in the absence of...

04 February 2019 3,597 3 View

Can I know how does centrifugation speed and time affect cell pellet compactness after the Falcon tube is removed from the centrifuge?

I used the parameters proposed by Yan et. al. Engineering Yarrowia lipolytica to Simultaneously Produce Li... (2000 g, 5 min) to and succeed to sediment Y. lipolytica cells, just that the...

13 December 2018 3,096 2 View

In non-oleaginous yeast, is there any evidence showing that there is no physical association between their ME and other fatty acid synthesis enzymes?

This is due to the fact that non-oleaginous yeast only have sources of NADPH from NAD:ICDH, G-6-PDH and 6-PGDH, but not ME, for their fatty acid biosynthesis. Hopefully research articles are...

05 October 2018 7,552 0 View