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Questions related from Lee Yee Seng
The recombination frequency of 2 unlinked genes is 50% (independent assortment, which is maximal achievable recombination frequency. For linked genes, their θ should be 0 < θ < 0.5 (not...
23 April 2020 7,600 0 View
I am having a problem when assaying ATP citrate lyase from the crude extract of Y. lipolytica (continuous assay) that is the exogenous activity is too high. Based on literature, I suspect it is...
19 February 2019 5,919 4 View
I am so confused as I found out that some sources mentioned Y. lipolytica can accumulate lipid up to 36% of DCW. But there are also some sources mentioned that the wild-type strain of the yeast...
12 February 2019 6,708 4 View
I am trying to extract lipid from Y. lipolytica. I had done sonication of the cells suspended chloroform-methanol (2:1). But after addition of 1% NaCl, some of the samples form bilayer...
10 February 2019 3,582 4 View
I am having a problem by assaying ATP-citrate lyase (ACL) continously. I had the validation with and without cell free extract (CFE) in my reaction mixture. The result is that in the absence of...
04 February 2019 3,591 3 View
This is due to the fact that non-oleaginous yeast only have sources of NADPH from NAD:ICDH, G-6-PDH and 6-PGDH, but not ME, for their fatty acid biosynthesis. Hopefully research articles are...
05 October 2018 7,549 0 View