Lee Yee Seng

4 Questions 5 Answers 0 Followers

Questions related from Lee Yee Seng

What is the suitable ammonium sulphate % for fractional precipitation of ATP-citrate lyase whereby that % doesnt precipitate complex I resp chain?

I am having a problem when assaying ATP citrate lyase from the crude extract of Y. lipolytica (continuous assay) that is the exogenous activity is too high. Based on literature, I suspect it is...

19 February 2019 5,917 4 View

Can I know why is Yarrowia lipolytica considered oleaginous even though its wild type strains rarely accumulate lipid more than 20% dry cell weight?

I am so confused as I found out that some sources mentioned Y. lipolytica can accumulate lipid up to 36% of DCW. But there are also some sources mentioned that the wild-type strain of the yeast...

12 February 2019 6,703 4 View

Hi all, can I know what are the possible reasons that there is no spontaneous formation of bilayer in folch method for lipid extraction?

I am trying to extract lipid from Y. lipolytica. I had done sonication of the cells suspended chloroform-methanol （2:1). But after addition of 1% NaCl, some of the samples form bilayer...

10 February 2019 3,579 4 View

Hi all, if I am to assay ATP-citrate lyase by coupling with malate dehydrogenase, can I add rotenone or amytal to enhance the accuracy of my assay?

I am having a problem by assaying ATP-citrate lyase (ACL) continously. I had the validation with and without cell free extract (CFE) in my reaction mixture. The result is that in the absence of...

04 February 2019 3,589 3 View