Leanna Surrao

4 Questions 4 Answers 0 Followers

Questions related from Leanna Surrao

What is the role of heating in DNA extractions?

I'm trying to develop crude methods of DNA extraction from whole blood and dried blood spots that don't require kits, many reagents, or centrifugation. The protocols I have found use heating in...

20 September 2016 184 5 View

Can anyone help with calcein detection of LAMP products?

I'm trying to use calcein as a detection dye for my LAMP assay, but am having trouble. I've tried titrating it out against MnCl2 and tried adding it before and after amplification but have only...

16 August 2016 1,137 21 View

Is it possible to contaminate a LAMP reaction after amplification?

I have been getting sporadic instances of contamination in my LAMP assay. Occasionally one duplicate in two negative controls will appear positive. As only one duplicate is contaminated, this...

16 August 2016 9,196 6 View

When designing LAMP primers, is the FIP primer (and similarly for BIP) made up of the F1c and F2 regions? If so what are the specifications for this?

I am trying to order some primers already published in the literature. The paper only has the sequence with the regions highlighted, but doesn't explicitly say what the FIP and BIP primers are. I...

09 June 2016 9,093 2 View