Law Mo

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Questions related from Law Mo

Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

19 October 2020 6,810 3 View

NHS reactive group on CyDye and iTRAQ reagents?

Both iTRAQ tags and DiGE CyDye have an NHS reactive group that reacts with primary amines. iTRAQ tags react with the epsilon amino group on lysine and the N terminal group of proteins where as...

02 September 2013 6,724 4 View